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In vitro shoot induction from cotyledon in Solanum lycopersicum L.
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Fatemeh Ghanei Estalkhi   , Shirin Rouzbahani , Raheleh Karimi-Ashtiyani * |
| Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran |
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Abstract: (322 Views) |
The tomato, Solanum lycopersicum L., is a self-pollinated perennial plant from the Solanaceae family. Due to its high consumption and importance as a model plant, there is a need to increase its production. Genetic engineering and editing can be used to produce tomatoes more efficiently while maintaining their desirable characteristics. To achieve successful editing in the genome of plants, an optimal protocol for the desired plant transformation and regeneration is necessary. In this research, the effect of cytokinin growth regulators BAP and Zeatin complemented with Auxin growth regulator and vitamins B5 and MS on shoot regeneration in tomato cv. Micro-Tom was investigated. The results showed that the T4 treatment including the hormonal combination of 0.1 mg l-1 IAA + 2 mg l-1 Zeatin and B5 vitamins was the most effective treatment used in the regeneration of tomato cv. Micro-Tom for the new shoot induction trait with an average of 6.54. The results obtained from this research can provide the basis for improving the efficiency of transformation in functional genomic studies of this tomato cultivar.
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| Keywords: Micro-Tom, Cytokinin growth regulators, Shoot regeneration, Transformation, Tissue culture |
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Type of Study: Research |
Subject:
Special
Received: 2024/02/11 | Accepted: 2024/03/9 | Published: 2024/03/10
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