For years, prescriptions medicinal was used for treatment of different fish, especially bacterial contamination, however, faced with the issue of bacterial resistance against antibiotics and the accumulation of these substances in fish breeding as well as the effects of emissions of these drugs on the environment was the most important problems of the pharmaceuticals in fish and other aquatic animals. Vitamin, as one of the main constituent parts of the feed, is necessary in food rations for survival, growth and natural reproduction of animals. There are 11 kinds of water-soluble and fat-soluble vitamin in the diet of fish and shrimp. Vitamin C is one of the most sensitive vitamins that has multiple metabolic roles, including effects on growth, survival and prevent mortality, improve injurues, reducing the effects of stress and resistance to pathogens and improve the performance of reproduction to crosses. Vitamin C plays an important role as an antioxidant to protect cells against oxidative damage. Oxidizing agents such as vitamin C as biomarkers can act as defense barrier against oxygen free practice groups and cause cells to collect and remove them from the action occurs through a trap off free radicals. The use of probiotics is a new technology of aquaculture technology sync with the environment. These materails are of the ways of preventive health management of fish farms and can consider as bilogic treatment. Based on studies it can be concluded that the addition of probiotics could be improved growth indices and immun response of aquatic animals.