Given this fact that conventional crop technology, lonely, cannot feed the immense growing number of world’ population, the global scientific community agreed on a balanced and simultaneous consumption of both conventional and biotechnological (GM and non-GMtraits) crop. Although twenty years’ commercialization of biotech crops has confirmed no negative impacts of GM plant on environment and living organisms, still risk assessments are required. Since the traditional analysis is able to recognize solely the intended effects, omics approaches are applied to determine the unintended effects. These nontargeted approaches have become more comprehensive in characterizing the composition and performance of GM crops and detecting the unintended effects. Therefore, Omics studies would provide important information for understanding changes in different biological levels after genetic modification and are important for evaluating biological safety of GM crops.