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:: Volume 13, Issue 1 (6-2020) ::
2020, 13(1): 87-110 Back to browse issues page
Exploring Plant Defense Responses to Pathogens by RNA-seq Technology
Fatemeh Khelghatibana * , Ahmad Sobhani Najafabadi , Kobra Moslemkhani
PhD in Plant Pathology, Iranian Research Institute of Plant Protection, Plant Protection Lab, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Abstract:   (2992 Views)
Unlike animals, plants do not have mobile defense cells and their immune systems are not naturally capable of developing and adapting to pathogens. The innate immune system of plants is very complex and organized in two important layers and extensively depends on signaling pathways. Each layer is composed of many components, which timely play their roles, chemical and physical barriers preventing pathogens or oxidative bursts, pathogen-related proteins, and programmed cell death that induced after pathogen recognition are all parts of plant complicated immune system. Transcriptome analysis of the plant pathogen interaction and listing differentially expressed genes (DEGs) shed light on the plant complicated responses to pathogens. The high throughput RNA-seq technology, developed after Next-generation sequencing arrival, is of the most used techniques in DEG analysis. Here, the basics and steps of the RNA-seq technique to study of the plant defense responses were summarized.
Keywords: Plant Immune System, Plant/Pathogen Interaction, RNA-seq, Paired end Reads, DEG Analysis.
Full-Text [PDF 2051 kb]   (469 Downloads)    
Type of Study: Research | Subject: Special
Received: 2020/11/2 | Accepted: 2021/01/18 | Published: 2021/02/3
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khelghatibana F, Sobhani Najafabadi A, Moslemkhani K. Exploring Plant Defense Responses to Pathogens by RNA-seq Technology. Journal of Biosafety 2020; 13 (1) :87-110
URL: http://journalofbiosafety.ir/article-1-356-en.html


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Volume 13, Issue 1 (6-2020) Back to browse issues page
فصل نامه علمی ایمنی زیستی Journal of Biosafety
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