DNA vaccines may be safer than of some live virus vaccines. Largely Immune response to the vaccine antigen correlates with expression level Which is directly based on transient transfection of cells in vitro measured DNA vaccines into muscle or skin inoculated with a syringe or a device such as a gene gun. DNA uptake by cells Followed by a pathogenic gene transcription and translation and subsequent immune response of antibodies (Ab), helper T cells (Th) and cytotoxic T lymphocytes (CTL) to follow The DNA vaccine are used for immunization against a variety of diseases, including viral infections, bacterial, parasitic, allergic, autoimmune and cancer. In some studies, DNA vaccines express multiple antigens. Even with this, the immune system is induced. Combinational vaccines increase protection against a single pathogen or protection against a range of infectious agents is induced by DNA vaccine. The aim of DNA vaccination development and improvement of effective strategies against effective infectious agents and tumors of the previous unifie.