The production of genetically modified organisms (GMOs) refers to crops, animals and microorganisms that emerged over the past three decades. GMOs triggered the opportunities to meaningfully increase the quantity and quality of production in agriculture, enhance nutritional quality, management of pests, weed and disease sustainability, reduce costs for food or drug production, improve public health as well as enhancing the quality of life. In following, systematic genetic dissection of modified organisms causes to dramatically expand the genomic knowledge and understanding. Based on the prevalence the transgenic organisms many countries or companies may need to execute GMO tests on diagnosing transgenic organisms and their products. The detection and labeling of transgenics crops aids researchers, farmers, suppliers, consumers, and GMO reference laboratories distinguish and track the genetic identity of GMOs at every stage of research, production, and transportation. The aim of the present review is to overview the antibody-based immunoassay methods for the detection of GMOs and their recombinant proteins.