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Transformation of FT Gene into Sunflower and Investigation of Its Effect on Flowering Tsime
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Maghsoud Pazhouhandeh *   , Mohsen Sajjadi Fard , Akbar Shirzad , Mohammad Ahmadabadi |
| Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran. |
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Abstract: (33 Views) |
Plants are dependent on flowering in a way that the flower is either the main purpose of the crop or is required for crop production. Flowering initiation (transition from the vegetative to reproductive phase) is an important and vital stage for the plant. Genetic studies on flowering have identified at least four important flowering pathways, including the photoperiod, the vernalization, the autonomous, and the gibberellin pathway. In the photoperiod pathway of flowering, the Flowering Locus T (FT) gene, under the influence of the CONSTANS (CO) gene, initiates the flowering process with increasing sunlight exposure. In this study, the cDNA of the FT gene of the Arabidopsis model plant was first amplified and isolated by PCR with the help of specific primers and cloned into the pMDC43 expression vector. After sequence confirmation, it was transferred to Agrobacterium cells and transferred to cotyledon and leaf segments of sunflower. The explants were induced to callus and plant, and independent transgenic plants were produced under selection with hygromycin at a concentration of 10 mg/l, then propagated and phenotypically examined in terms of flowering time. DNA extraction was performed from the transgenic plants and the presence of the transgene in them was confirmed by PCR technique using Ca35S promoter specific primers. Transgenic plants showed earlier flowering than non-transgenic plants. The results of this study showed that by transformation of the FT gene, flowering in sunflower can be accelerated and the crop can be ripened earlier.
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| Keywords: Sunflower, Transformation, Tissue Culture, Flowering, FT gene |
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Type of Study: Research |
Subject:
Special
Received: 2025/08/24 | Accepted: 2025/12/14 | Published: 2025/12/27
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