Journal of Biosafety
ايمني زيستي
Journal of Biosafety
Basic Sciences
http://journalofbiosafety.ir
1
admin
2717-0632
2716-9804
4
4
fa
jalali
1402
6
1
gregorian
2023
9
1
16
2
online
1
fulltext
fa
نوساقه زایی درون شیشهای از ریزنمونه لپهای در گوجهفرنگی (Solanum lycopersicum L)
In vitro Shoot Induction from Cotyledon in Solanum lycopersicum L.
تخصصي
Special
پژوهشي
Research
<div style="text-align: justify;"><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">گوجهفرنگی </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">(<i>Solanum lycopersicum</i> L.)</span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">گیاهی چندساله و خودگشن از خانواده بادنجانیان است. به دلیل مصرف فراوان و اهمیت آن بهعنوان یک گیاه مدل، نیاز به تولید این گیاه رو به افزایش است. از اینرو، رویکردهای مهندسی ژنتیک و ویرایش ژنومی می‏تواند در جهت تولید بیشتر و کارآمد گوجهفرنگی توام با حفظ ویژگیهای مطلوب آن کارآمد باشد. داشتن دستورالعمل بهینه انتقال ژن و باززایی در گیاهان جهت انجام موفقیتآمیز ویرایش ژنومی اجتنابناپذیر است. در این پژوهش، اثر تنظیمکنندههای رشد سیتوکینین </span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">BAP</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">و </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">Zeatin</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">در ترکیب با تنظیمکنندهی رشد اکسین</span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">IAA</span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">و ویتامین‏های محیط کشت </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">B5</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">و </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">MS</span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">در محیط کشت پایه </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">MS</span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">بر روی باززایی نوساقه در رقم</span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">Micro-Tom</span></span></span></span></span></span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">گوجهفرنگی بررسی شد. نتایج نشان داد که تیمار</span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR" lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" times="">T4</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">شامل ترکیب هورمونی </span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">IAA (0.1 mg l<sup>-1</sup>)</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">Zeatin (2 mg l<sup>-1</sup>) + </span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">و ویتامین‏های </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-size:12.0pt"><span b="" lotus=""> </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">B5</span></span><span style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">برای صفت القاء نوساقه با میانگین </span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-size:12.0pt"><span b="" lotus=""> 6/54 </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">موثرترین تیمار مورد استفاده در باززایی رقم </span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-size:12.0pt"><span b="" lotus=""> </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" times="">Micro-Tom</span></span></span></span></span></span></span><span style="font-family:IRANsans;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:12.0pt"><span b="" lotus=""> </span></span></span></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus"">گوجهفرنگی بود. نتایج بهدست آمده از این پژوهش می‏تواند زمینه را برای بهبود کارایی انتقال ژن در مطالعات ژنومیک عملکردی این رقم گوجهفرنگی فراهم سازد.</span></span></div>
<span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">Tomato, <i>Solanum lycopersicum</i> L., is a self-pollinated perennial plant from the Solanaceae family. Due to its high consumption and importance as a model plant, there is a need to increase its production. Genetic engineering and editing can be used to produce tomatoes more efficiently while maintaining their desirable characteristics. To achieve successful editing in the genome of plants, an optimal protocol for the desired plant transformation and regeneration is necessary. In this research, the effect of cytokinin growth regulators BAP and Zeatin complemented with Auxin growth regulator and vitamins B5 and MS on shoot regeneration in tomato cv. Micro-Tom was investigated. The results showed that the T4 treatment including the hormonal combination of 0.1 mgL<sup>-1</sup> IAA + 2 mgL<sup>-1</sup> Zeatin and B5 vitamins was the most effective treatment used in the regeneration of tomato cv. Micro-Tom for the new shoot induction trait with an average of 6.54. The results obtained from this research can provide the basis for improving the efficiency of transformation in functional genomic studies of this tomato cultivar.</span></span>
Micro-Tom, تنظیمکنندههای رشد سیتوکینین, القا نوساقه, انتقال ژن, کشت بافت
Micro-Tom, Cytokinin Growth Regulators, Shoot Regeneration, Transformation, Tissue Culture
1
16
http://journalofbiosafety.ir/browse.php?a_code=A-10-821-1&slc_lang=fa&sid=1
Fatemeh
Ghanei Estalkhi
فاطمه
قانعی استلخی
g.fatemeh@modares.ac.ir
10031947532846003583
10031947532846003583
No
Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران
Shirin
Rouzbahani
شیرین
روزبهانی
shirin.rouzbahani@modares.ac.ir
10031947532846003584
10031947532846003584
No
Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران
Raheleh
Karimi-Ashtiyani
راحله
کریمی آشتیانی
r.karimi@modares.ac.ir
10031947532846003585
10031947532846003585
Yes
Assistant Professor, Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران