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:: Volume 10, Issue 3 (11-2017) ::
2017, 10(3): 135-152 Back to browse issues page
Applications, alternatives and biosafety of markers in transgenic plants
Masoud Tohidfar * , Mohssen Hosseini
Associate Professor, Department of Biotechnology, Faculty of Engineering and New Technologies, Shahid Behesgti University, Teharn, Iran
Abstract:   (3476 Views)
Selectable marker (SM) systems have been considered necessary to achieve acceptable rates in the generation of Genetic Modified (GM) plants. These systems allow the relatively straightforward identification and selection of plants that have stably incorporated not only the marker genes but also genes of interest. Since the creation of the first generation of GM, genes encoding resistance to specific antibiotics and herbicide have been largely used for selection and rapid identification of transformed cells. In most cases, once transgenic plants have been regenerated, permanence of SM genes in the plant genome is no longer necessary, and it becomes a matter of public concern, although no evidence of adverse biosafety effects has been found associated with marker genes. A valuable substitute to the SM genes are the reporter genes, which encode proteins whose activity can be easily identifiable, or make it possible to easily screen GM plant. In addition to replacement of marker genes with reporter, other methods such as co-transformation, site-specific recombination of FLP / FRT, Cre / lox, R / RS and lossmarker can be used to remove marker genes from transgenic plant. In the current paper, we present a list of SM that are widely used for the production of transgenic plants, their replacement, elimination strategies and biosafety are presented.
Keywords: Selectable marker, Genetic Modified, Biosafety, Reporter, Transformation
Full-Text [PDF 1059 kb]   (2526 Downloads)    
Subject: Special
Received: 2017/06/23 | Accepted: 2017/11/14 | Published: 2019/02/28
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Tohidfar M, Hosseini M. Applications, alternatives and biosafety of markers in transgenic plants. Journal of Biosafety 2017; 10 (3) :135-152
URL: http://journalofbiosafety.ir/article-1-190-en.html


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Volume 10, Issue 3 (11-2017) Back to browse issues page
فصل نامه علمی ایمنی زیستی Journal of Biosafety
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