Nematodes are one the most economically important groups of plant pathogens. The use of resistant or non-host crop plants, application of nematicides and more recently the use of microbial antagonists and biocontrol agents are the principal methods for management of nematodes. Taking advantage of nematode repellents, Proteinase inhibitors, Cry proteins of Bacillus thuringiensis, resistance genes, RNA interference (RNAi) etc. are among the novelest methods of management. The use of host plant resistance against nematodes is limited because of low numbers of identified plant species that bearing resistance genes. Along with other control methods, RNA silencing is a novel tool to control of plant parasitic nematodes. RNAi is a cellular mechanism in which double-stranded RNA (dsRNA) molecules drive the post-transcriptional silencing of genes with homologous sequences. RNAi on parasitic nematodes is generally performed by soaking of nematodes in dsRNAs or siRNAs of target genes. Three main target genes of nematodes are house-keeping genes, parasitism or effector genes and the genes necessary for development of nematodes. A combination of the RNAi technology with existing technologies or treatments might provide the most effective and durable basis for future control of these important plant parasitic nematodes.