In the fisheries industry, compared to other food industries, a large volume of by-products such as bone, skin, and viscera are produced, which are costly to treat and dispose of in the environment. The rapid growth of tilapia and its marketability have also been considered. The value of proteins as the main source of amino acids in human health has been proven. In addition to their nutritional value, proteins have biological functions. These proteins show their functions through bioactive peptides. Production methods of bio peptides affect their biological activity. The articles showed that the use of enzymatic hydrolysis method for therapeutic applications makes them more stable than fermentation and solvent extraction methods and is a safer method than the other two methods. Bioactive peptides have physiological effects such as antihypertensive, antioxidant, antimicrobial, anti-cancer, anti-diabetic, etc. and have many applications in the food, pharmaceutical and cosmetic industries and can be added to the food industry. The purpose of this article is to review the extraction of bioactive peptides from fish waste.