Potyviruses are one of the largest families of plant viruses (Potyviridae) with the most economic importance. Potyviruses have flexuous filaments and the genome consists of a positive sense single stranded RNA with 183 species. Today, biological, serological, and molecular assays are used to screen potyviruses. In this review, the application of different methods is described to the identification of potyviruses that are infected with a wide range of plants in Iran and the world. The use of primers, especially degenerate primers, has not only facilitated the rapid detection of potyviruses but also the sequencing of a part of the genome also makes the taxonomy of these viruses possible. Today, the TaqMan system has been developed and can show the number of early RNA copies of potyviruses in infected plants compared to healthy plants. Transcriptome technology demonstrates complex reactions between putative multifunctional proteins and their association with host proteins. This method is involved in the detection of molecular and physiological responses of infected plants with potyviruses as well as their evolution and phylogeny. CRISPR technology is one of the effective and novel methods for purposeful editing of the genome that is used to improve the quality and yield of plants and create new characteristics in them. This method can be used to produce resistant plants to potyviruses.