Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Abstract: (1965 Views)
Micrografting is a grafting technique that involves the placement of a small meristem or section of shoot-tip onto a decapitated rootstock that has been grown aseptically from in vitro seedlings. In vitro micrografting provides many benefits to producers in agriculture and natural resources. The success rate in micrografting can be affected by different factors such as cultivar or genotype, scion length and rootstock development stage and adaptation of a micrografted seedling in greenhouse conditions. In this review, various applications of micrografting techniques such as early detection of viral, viroid and phytoplasma infections, obtaining of grafted disease-free plants free from virus and virus-like disease, overcoming ecological limits, providing an alternative production technique, rejuvenation of mature tissue, bypassing the juvenile phase in fruit trees, recovery of cryopreserved shoot tips, allowing a quick supply of safe germplasm exchange, facilitating the early diagnosis of grafting incompatibilities, saving rootless shoots and deficient embryos, testing long-distance signalling in plants, productions of polyploidy plants are discussed.