Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Abstract: (1494 Views)
Targeted genome editing technology is becoming one of the most important genetic tools widely used in plant pathology. In recent years, CRISPR (Clustered regularly interspaced short palindromic repeat) and CRISPR-related proteins discovered in prokaryotes immune systems have been successfully used in various genome editing tools due to their simplicity, high efficiency, and versatility. The CRISPR/Cas system, with targeted, precise, and strong genome modification, has improved the crop and paved the way for new methods of improving the genetic resistance of plants against plant diseases. This review provides an overview of the CRISPR/Cas9 system, derived tools, and their applications in plant pathology. Also, the creation of resistance against bacterial, fungal, and viral pathogens in the host using CRISPR/Cas-based tools is described and the limitations of the CRISPR-Cas9 system, the regulation of products edited with CRISPR-Cas9, and the future of this technology are discussed.
Ghermezi Nejad M, Shahryari F, Aali Mohammadi A. Application of CRISPR/Cas9 System in Plant-Pathogen Interaction. Journal of Biosafety 2023; 15 (4) :47-72 URL: http://journalofbiosafety.ir/article-1-504-en.html