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The Development of a Composite Substrate of Polymer and Gold Nanoparticles, Integrated with Aptamers, Aimed at the Capture of Salmonella Bacteria from Aquatic Environment
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Ehsan Shokri *   , Maryam Asnaashari , Najmeh Nasiri , Saeed Soheilivand |
| Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. |
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Abstract: (602 Views) |
The implications of salmonella contamination extend significantly across both economic and public health domains at both national and global scales. Consequently, the ongoing surveillance and assessment of Salmonella presence in water, food products and environmental contexts are crucial for ensuring food safety and enhancing biosecurity measures. Regrettably, the prevalence of negligence and the limited availability of affordable and straightforward detection techniques for Salmonella, particularly in middle- and low-income nations, contribute to excessive and inappropriate utilization of antibiotics and chemicals within healthcare and agricultural sectors, including livestock, poultry, and aquaculture. Presently, the rise of antibiotic resistance among certain invasive and harmful organisms renders salmonella a considerable biosecurity concern. This study explored the feasibility of isolating Salmonella bacteria utilizing a laboratory-developed absorbent substrate. The findings indicated that a 2% agarose polymer film infused with 40% glycerol and bio-activated with gold nanoparticles linked to an aptamer can effectively isolate Salmonella bacteria from water samples. This innovative separation technique has the potential to enhance current diagnostic practices while simultaneously reducing both costs and the duration of investigations. |
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| Keywords: Aptamer, Biosecurity, Composite, Salmonella Pathogen. |
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Full-Text [PDF 1514 kb]
(93 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/11/12 | Accepted: 2024/11/22 | Published: 2024/11/23
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