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Cryopreservation of Satureja spicigera seeds
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Leila Ghafarzadeh namazi *   , Nadali Babaeian , Abas Ghamari-zare , Ghorbanali Nematzadeh |
| Plant Breeding at Sari Agricultural Sciences and Natural Recourses University |
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Abstract: (3305 Views) |
| In recent years, our country has drawn an increasing amount of attention to medicinal plants. Because different plant species Savory inappropriate harvesting of natural areas by humans, and the growing threat of biotic and abiotic stresses following the work resulted in reduced genetic diversity of a species is, a species of genetically keep reserves Curb upper's. Seed storage techniques using ultracold temperatures is one way of maintaining germplasm habitat conditions are outside, you can seed a long time, with much less cost and without loss of viability on storage. Using seed cryopreservation, as one of the Ex situ plant germplasm conservation method we can store seed for long-term, with much lower expenses and without losing seed viability. In order to preserve Satureja spicigera seed under -196ºC condition, three pre-cryopreservation treatments, PVS2 solution, Desiccation and Glycerol were applied. The treated seeds were transferred into Liquid Nitrogen (LN) and stored for 7, 30 and 90 days respectively. In this study the effect of cryopreservation on germination and growth indices (germination percent, germination rate, seed vigour index, plantlet length on orthodox seeds of plant species (Satureja spicigera) in storage conditions of cryopreservation (-196ºC) were evaluated for 7, 30 and 90 days. Comparing treatments, significant differences were observed on germination indices and germination rate. The results showed that, the long-term preservation of the species, seed in -196 ºC using suitable precryopreservation treatment is possible. |
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| Keywords: atureja, Cryopreservation, Germplasm, Glycerol, Vitrification |
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Full-Text [PDF 704 kb]
(1276 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2014/05/20 | Accepted: 2019/02/8 | Published: 2019/02/8
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