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Using the technology of Sleeping Beauty transposons for genetic engineering and cell-mediated immunity
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Naghmeh Gholipour *   , Malihe Naderi , Amir Mousavi , Fatemeh Akbarian , Simin Nafian dehkordi , Farzaneh Khani , Fatemeh Nafian dehkordi |
| National Institute of Genetic Engineering and Biotechnology, Tehran, Iran |
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Abstract: (3841 Views) |
| Sustainable production of transgenes in human cells can be combined of both viral and non-viral methods for gene therapy. Efficient transfection into primary cells is proposed by recombinant virus technology; even though provided by reports of successful treatment, it still has a lot of disadvantages. As of today, the use of plasmids can be a viable alternative to viral vectors for its reduced costs, low immunogenicity, and effective insertion. In the past decades, Sleeping Beauty transposons (SB) have been developed as a non-viral vector system for gene therapy, with the shared benefits of both vectors and naked DNA. The SB system has been successfully used in human T cells for generating specific chimeric antigen receptors (CAR). This article is aimed to introduce transposon technology for a safe genetransfer into human cells with the emphasis on SB systems. Moreover, viral and non-viral gene transfer systems are described by considering both the advantages and disadvantages. |
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| Keywords: Transposon system, Sleeping Beauty transposon, nonviral vector, gene therapy, biosafety. |
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Full-Text [PDF 709 kb]
(2546 Downloads)
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Type of Study: Applicable |
Subject:
Special
Received: 2014/06/27 | Accepted: 2019/02/24 | Published: 2019/02/24
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