While Nanotechnology is providing exponentially new products based on Nano tools, equipment and nanoparticles, the study of their toxicity would be essential. The reduction in size to Nano scale leads to change their surface properties and also increases their chemical activity that causes toxicity. The knowledge of Nano particles entrance mechanism to the body is useful in evaluation of nanoparticle content in the cell therefore we are able to answer some of the questions about toxicity. A variety of Nanoparticles have different size, shape, surface and chemical composition and they have different toxicity mechanism in the interface with living systems. We will focus to the interaction of nanoparticles with cells, blood and immune system and finally we will briefly express the steps of toxicity identification of nanomaterial.