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Development of Forest Trees Resistant to Biotic and Abiotic Stresses Using Advanced Genome Editing Technologies
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Zeinab Bahmani *   , Amirreza Paknzhad , Zahra Mirsoleymani |
| Ilam University |
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Abstract: (11 Views) |
Conventional genetic improvement methods in forest trees face significant limitations, including extended breeding cycles and low efficiency in enhancing desirable traits. These challenges have reduced their effectiveness in meeting the growing demands of forest sustainability. As a result, the use of modern biotechnological tools has become increasingly important. This article introduces several emerging approaches, such as Agrobacterium-mediated transformation, particle bombardment, nanoparticle-based delivery, CRISPR/Cas genome editing systems, and peptide aptamers. Some of these methods—like base editing and spray-induced gene silencing (SIGS)—allow for precise, non-transgenic modifications without introducing foreign DNA. These technologies aim to improve tree tolerance to a range of biotic and abiotic stresses, including pests, diseases, drought, salinity, and low temperatures. While offering promising solutions, they also raise concerns regarding biosafety, the long-term stability of modified traits in natural environments, and the availability of advanced research infrastructure. Nevertheless, when applied thoughtfully and responsibly, these tools can contribute meaningfully to biodiversity conservation, forest regeneration, and adaptation to climate change, especially in ecologically fragile regions. This review highlights both the opportunities and challenges associated with these technologies and underscores their potential role in the future of sustainable forest breeding and management programs.
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| Keywords: Peptide aptamers, Genetic improvement, Genetic manipulation, gene silencing, nanoparticles |
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Type of Study: Review |
Subject:
Special
Received: 2025/08/7 | Accepted: 2025/12/15
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