The software implements standard statistical approaches commonly adopted in validation studies to estimate analytical method performance (limits of detection and quantitation, precision and accuracy). This software can also be used for tests to determine the normal distribution of data, to check the homogeneity of variances as well as a choices of method selection according to specific user needs. The importance of validation of analytical methods have been risen. Validated methods for the detection of genetically modified organisms (GMO) are requested by European union (EU) legislation in order to accept GMO crops on the European market. The AMPE software could be used to synchronize and standardize the results of the assessment of GMO in different countries and the competent authorities. In most cases, validation requires the assessment of the performance of the proposed method by means of an inter laboratory studies. This application has been developed based on international protocols ISO, Codex CCMAS and the ISO 5725 (1994). This software has to compare different methods and evaluate their adequacy with respect to specific needs, based on summarized information collected during the validation and recognition GMO crops.