Orange is with academic name of Citrus aurantium from Rutaceae family and citrus of Citrus. In order to study the germination, regeneration, and root of orange (Citrus aurantium L.) under in vitro conditions, orange primers containing hypocotyl and epicotyl fragments were seeded for four weeks in the dark and then for seven days in Lighting was provided. These explants were cultured in MS medium supplemented with four levels of kinetin growth regulators (0, 0.5, 1 and 2 mg / L) and indole acetic acid (IAA) (0, 0.5, 1 and 2 mg / L). The highest level of germination was observed at 0.5 mg / L Kuintin, and then 2 mg / L IAA in addition to 0.5 mg / L Kinetin, which indicates that IAA hormone is not effective for increasing germination. In rooting section, the effects of strains 15834, 9534 and 318 Agrobacterium rhizogenesis, also the concentration of 2 mg / L of NAA growth regulator on rooting of orange citrus (citrus aurantium) strains were investigated. Among the treatments, NAA and strain 318 combination treatments were the most suitable treatments for traits in this study.