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Prime Editing: A New Step toward Reducing Biosafety Concerns in Plant Genome Editing
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Sedigheh sadat Seyeddoaeian   , Ebrahim Dorani * |
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Abstract: (16 Views) |
Rapid population growth and climate change have made the development of high-yielding, stress-tolerant crops a global necessity. The emergence of genome editing technologies such as CRISPR-Cas9 has revolutionized this field. However, biosafety concerns arising from DNA double-strand breaks and off-target effects have posed obstacles to their application in plants. Prime editing, as a next-generation genome editing tool, can introduce various types of mutations without inducing double-strand breaks and with significantly reduced off-target effects, and it does not require an external DNA donor template. Therefore, despite certain limitations of Prime editing, such as its low efficiency in plants and PAM sequence constraints, this technology holds great potential for the development of superior agricultural crops for various purposes, including yield enhancement, conferring resistance to various abiotic and biotic stresses, and improving the quality of plant products. Accordingly, this article aims to provide a brief introduction to this technology as a novel step toward mitigating biosafety concerns associated with genome editing technologies in plants.
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| Keywords: Food security, Genetic modification, CRISPR, Genome editing |
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Full-Text [PDF 1068 kb]
(7 Downloads)
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Type of Study: Review |
Subject:
Special
Received: 2026/05/4 | Accepted: 2026/05/9
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