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DNA sequencing and detection of bio molecules with Nanopores
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Behnam Derakhshani *   , Khadijeh Bagheri |
| Biotechnology, Faculty of Agriculture, Zanjan University, Zanjan, Iran |
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Abstract: (4165 Views) |
| Oxford Nanopore system, as the third-generation DNA sequencing technology, are being developed for fast and direct sequencing of single DNA molecules by detection of ionic current modulations as DNA passes through a pore. Moreover, the single molecule techniques used by this technology allow us to identify proteins, MicroRNAs as well as their concentrations. Oxford Nanopore systems open a new door to molecular biology investigation at the single-molecular level. Due to the blockades of ionic current, nanopores can help us to get information about the identity, concentration, structure and dynamics of target proteins and miRNAs. The Oxford Nanopore has introduced a new device namely MinION, with the size of a USB memory stick, which can sequence the entire human genome for less than $1000. High speed, sensitivity in detection of single nucleotides and the ability of reading long sequences of DNA molecules have made the nanopores as promising technology in high throughput sequencing. Since the lack of proper Persian language resources, the authors intend to introduce this technique for researchers. |
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| Keywords: Oxford Nanopore, Third generation sequencing, MinION, miRNA. |
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Full-Text [PDF 985 kb]
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Subject:
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Received: 2015/08/17 | Accepted: 2019/02/20 | Published: 2019/02/20
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