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Investigating the diversity of phage and plasmid targets of the CRISPR/Cas systems in the Leuconostoc genus using the insilico approach
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Sara Ghaffarian *   , Bahman Panahi |
| 1- Assistant Professor, Department of Cellular and Molecular Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. |
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Abstract: (1175 Views) |
Leuconostoc genus bacteria are part of the natural microbial flora of fields and fermented products. CRISPR/Cas system is one of the main parts of the bacterial immune system. In this study, the Leuconostoc genus various strains' evolutionary pathways based on composition, acquisition, and deletion events of spacer sequences under selective pressure were evaluated and the diversity of invasive phages and plasmids targeted by the CRISPR/Cas system of Leuconostoc strains was studied. The maximum similarity in terms of composition, acquisition, and deletion events similar pattern between strains within specious detected in Leuconostoc gelidum and Leuconostoc pseudomesenteroides. Forty-five spacers of strain AMKR21 from L. gelidum matched Alteromonas phage JH01 which harbored the highest numbers of spacers targeting phages. Also, the highest number of identified phages by Leuconostoc belonged to Escherichia phages. A plasmid of Leuconostoc citreum was the most frequently targeted plasmid by the analyzed spacers of Leuconostoc and it was matched 177 times by spacers of NBRC113246 from L. gelidum. The results of this study showed the importance of the bacterial adaptive immune system in stability in bacteriophage-contaminated environments and the usage of this information in defining functional formulations. |
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| Keywords: Leuconostoc genus, Adaptive immune system, Genetic diversity, Evolution |
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Full-Text [PDF 3714 kb]
(227 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/07/15 | Accepted: 2023/09/3 | Published: 2023/09/11
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