Biofertilizers, as one of the environmental innovations, significantly improve soil quality and enhance nutrient uptake efficiency by utilizing microbial compounds, including Plant Growth Promoting Bacteria (PGPB) and symbiotic fungi. Scientific studies have shown that these fertilizers significantly reduce the accumulation of nitrogenous compounds and heavy metals in agricultural products compared to chemical fertilizers. The functional mechanisms of biofertilizers include activities such as nitrogen fixation, dissolution of mineral phosphates, and the production of compounds that stimulate plant growth. Research also indicates the role of these fertilizers in enhancing soil microbial diversity and improving its physicochemical properties, such as cation exchange capacity and organic matter content. The present article introduces various types of biofertilizers, examines the environmental impacts of this technology, and offers suggestions for optimizing its application to promote sustainable agriculture and biological conservation. Findings indicate that the use of biofertilizers not only prevents pollutants from entering the food chain but also ensures the health of agricultural ecosystems.