Natural pesticides are eco-friendly and effectively eradicate pests without leaving a toxic effect or harm to environment. However natural pesticides have a low biodegradable and low threat to non-target organisms, such as humans, but they quickly degradate because of their instability to environment condition such as light. In the present study, it has been attempted to improve the stability of the active ingredient using nanotechnology. Botanical Neem oil-based nanopesticide was produced in the Agricultural Biotechnology institute of Iran (ABRII). Then, the effect of nanopesticide on the greenhouse whitefly pest at the nymph stage of age 2 and also its repellency effect on adult whitefly insects were investigated. The obtained results showed that the botanical nanopesticide with a maximum concentration of 200 mg.L^-1 has an effect of more than 95% on the control of whitefly via preventing the growth of 2nd nymphs and the formation of pupae. The application of nanotechnology-based formulations can be effective and promising to improve the efficiency of natural pesticides, and further research needs to be done.