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Mutagenesis by Nitrosoguanidine and UV to Increase Coenzyme Q10 Production by Zymomonas mobilis
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Foozieh Moghadami *   , Leila Shokrzadeh |
| Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran. |
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Abstract: (915 Views) |
The present study investigated the increase of coenzyme Q10 production by the mutation in Zymomonas mobilis. For mutagenesis, the survival curve was first drawn using nitrosoguanidine and UV. Then the mutation was performed using a concentration of 3 mg/l of nitrosoguanidine and the UV exposure time of 60 seconds. Mutants were screened through their ability to grow in a medium containing 160 μg/ml menadione. Then the mutants were examined for coenzyme Q10 production. The results showed that the amount of coenzyme Q10 produced by Zymomonas mobilis was 8.4 mg/l and its dry cell weight was 1.3 g/l. Among 142 mutants, 16 strains were randomly selected. The highest amount of coenzyme Q10 was produced by the U7 strain, 11.9 mg/L, which was 1.4 times more than the parent strain. According to the results of this study, it could be concluded that the mutants can produce more coenzyme Q10 than the parent strain. |
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| Keywords: MMutation, Zymomonas, Coenzyme Q10, Nitrosoguanidine, UV |
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Full-Text [PDF 1727 kb]
(111 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/04/27 | Accepted: 2023/06/21 | Published: 2023/06/21
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