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Optimization of Proliferation, Callus Formation and Indirect Organogenesis of Moringa peregrina
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Farzad Banaei-Asl *   , Hamed Salehian |
| Assistant Professor, Biotechnology Research Department, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. |
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Abstract: (1208 Views) |
Moringa peregrina, commonly known as Gazrokh, is one of the forest elements in the southern and southeastern regions of Iran. Due to its valuable nutritional and medicinal properties, this plant is considered highly valuable. The leaves, seeds, and roots of this plant contain vitamins, minerals, essential antioxidants, and various secondary metabolites. Despite its high economic, environmental, nutritional, and medicinal values, the propagation of this plant has faced challenges due to drought, habitat destruction, and consumption of its seeds as food and feed. Therefore, optimizing the tissue culture process is essential to facilitate and accelerate the proliferation of this plant. After collecting the seeds, they were cultivated under sterile conditions. The sterilized samples were cut into small pieces containing one nodule and no nodule (internode) (0.5 to 0.1 cm), and the explants were used for callus formation and indirect regeneration of callus the traits were measured. A combination of two growth regulators, BAP (0.5 to 1.0 mg/L) and NAA (0.1 mg/L), in MS basal medium, resulted in the maximum branch production from stem nodes. A combination of 2,4-D, NAA, and kin at concentrations of 0.5, 0.2, and 0.1 mg/L respectively induced callus formation. A combination of BAP, TDZ, and NAA at concentrations of 3.0, 0.5, and 0.1 mg/L respectively led to indirect organogenesis. The findings of this study will be useful for researchers, environmental conservation organizations, and individuals interested in cultivating and propagating M. peregrina for its numerous benefits. |
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| Keywords: Proliferation, In vitro, Tissue culture, Gazrokh, Moringa. |
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Full-Text [PDF 1510 kb]
(268 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/09/26 | Accepted: 2023/10/5 | Published: 2023/10/5
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