Assistant Professor of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Abstract: (752 Views)
Plant pathogens are a considerable risk to global food security due to the drastic reduction in yield and quality of crops. The deployment of RNA interference (RNAi) machinery for crop protection against plant pathogens agents is showing promising results. It is well set up that small RNA molecules, such as small interfering RNAs (siRNAs) and microRNAs (miRNAs), are included in regulating gene expression, and the process of RNA silencing at both transcriptional and post-transcriptional stages. Using host-induced gene silencing (HIGS), plants are genetically modified to produce small RNAs that infiltrate pathogen cells and deactivate specific genes. Alternatively, spray-induced gene silencing (SIGS) involves directly applying small RNAs onto plants, where they penetrate the pathogens and suppress target genes. This study delves into the roles of small RNAs and examines their potential application in combating a many plant pathogens through either HIGS or SIGS strategies providing an evaluation of their respective benefits and limitations.