Assistant Professor, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Abstract: (202 Views)
The "Lime Tree Witches' Broom Disease" is a deadly and quarantined disease that poses a significant threat to lime tree orchards in southern regions of Iran. The disease can be transmitted to healthy lime trees through various methods, including insect vectors, infected scions, and the use of contaminated tools. Unfortunately, due to its quarantined nature, many research and testing opportunities are limited in the field and open environment. Therefore, many studies must be conducted in isolated and controlled locations such as growth chambers and greenhouses to perform initial scientific research on the interaction between the pathogen and the plant, factors contributing to disease progression, testing various control and management methods, examining disease-tolerant plant varieties, and testing diagnostic kits in these environments. This research aims to find the best conditions for producing plants infected with the phytoplasma disease, which can be used in subsequent studies. In this study, we used infected and healthy grafts (as controls) with two types of grafts: the inverted T-budding and the terminal. The results indicated that the inverted T-budding had higher success rates compared to the terminal. This was determined by evaluating the grafting conditions and characteristics.
Soheilivand S, Safarnegad M. Investigating the Optimal Transmission of Phytoplasma Pathogen via Infected Scion to Mexician lime for Phytopathological Studies. Journal of Biosafety 2023; 16 (3) URL: http://journalofbiosafety.ir/article-1-545-en.html