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Investigating of Optimal Conditions for the Transmission of Phytoplasma Disease Agent Using Infected Scions on Mexician Lime Seedling for Phytopathological Studies
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Saeed Soheilivand *   , Mohammadreza Safarnegad |
| Assistant Professor, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. |
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Abstract: (430 Views) |
The lime tree witches' broom disease is a lethal and quarantine-regulated disease that poses a significant threat to lime orchards in southern Iran. The disease can be transmitted to healthy lime trees through various methods, including insect vectors, infected scions, and the use of contaminated tools. Unfortunately, due to its quarantine status, extensive research and testing opportunities in open field conditions are limited. Therefore, many scientific studies must be conducted in isolated and controlled locations such as growth chambers and greenhouses to perform initial scientific research on the interaction between the pathogen and the plant, factors contributing to disease progression, testing various control and management methods, examining disease-tolerant plant varieties, and testing diagnostic kits in controlled environments. The objective of this research is to find the best conditions for producing plants infected with the phytoplasma disease, which can be used in subsequent studies. In this study, both infected and healthy scions (as controls) were utilized with two types of T-budding (inverted T) and terminal grafting. By analyzing grafting conditions and the characteristics of rootstocks and scions, it was determined that budding grafts were more successful than terminal grafts in transferring the disease. |
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| Keywords: Scions, Rootstock, Witches' Broom Disease, Disease Transmission. |
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Full-Text [PDF 1627 kb]
(61 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/06/4 | Accepted: 2024/06/22 | Published: 2024/06/30
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